Metabolic Lysis of Yeast Protoplasts
نویسندگان
چکیده
منابع مشابه
Surface structure of yeast protoplasts.
The fine structure of the yeast cell wall during protoplast formation was studied by means of phase-contrast microscopy and the freeze-etching technique. The freeze-etching results indicated that at least in some cases the entire wall substance was not removed from the surface of the protoplasts. After a treatment of 30 min to 3 hr with 2% snail enzymes, an innermost thin wall layer as well as ...
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Hexachlorophene was found to be both a lytic and a fixative agent for protoplasts isolated from Bacillus megaterium. Concentrations of 50 to 100 mug of drug per mg of original cell dry weight were required to lyse 4.4 x 10(9) protoplasts (2 mg of original cell dry weight). At higher drug concentrations, protoplasts became fixed against osmotic stress and reduced in sensitivity to disruption by ...
متن کاملPropionate induced lysis of protoplasts of Bacillus megaterium.
Previous studies (Storck and Wachsman, 1957; Weibull, Beckman, and Bergstrom, 1959; Wachsman, Fukuhara, and Nisman, 1960) on enzyme localization were performed with cell fractions, obtained by lysing protoplasts in media of low osmotic pressure. In an attempt to determine the effect of the tonicity of the lysing medium on the distribution of enzyme activities between the membrane containing and...
متن کاملReversible Metabolic Swelling of Bacterial Protoplasts* T
In a previous communication (I) it was reported that the cell walls of Streptococcus fecalis (ATCC 9790) were susceptible to digestion by lysozyme. This finding subsequently allowed the preparation of protoplasts from these organisms by treatment of whole cells with lysozyme and the use of high concentrations of sucrose as the impermeantl substance necessary for osmotic stabilization. Further i...
متن کاملRegeneration of yeast protoplasts prepared using Novozym 234.
Novozym 234 (Novo lndustri A/S, Bagsraerd, Denmark) is a comparatively cheap and highly effective commercial preparation, derived from the fungus 7richoderma harziunum, which is used for the enzymic degradation of the cell wall in many yeasts and filamentous fungi in order to release protoplasts [ 1 , 21. The factors involved in protoplast isolation using this system have been investigated by C...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1968
ISSN: 0022-1287
DOI: 10.1099/00221287-51-3-433